Figure 1. EasySep&#; Total Nucleic Acid Extraction Kit Workflow
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Diagram of the standard extraction workflow. Time points at which samples must be placed in the magnet are indicated with gray boxes. EasySep&#; Lysis Buffer and Proteinase K are added to the sample and incubated at 56°C for 10 minutes. Diluted EasySep&#; Nucleic Acid RapidSpheres&#; are added to the sample and incubated at room temperature (RT) for 5 minutes, then placed in the magnet for 2 minutes. While in the magnet, the sample is washed three times with a 70% ethanol wash solution, and the supernatant is removed. The pellet is resuspended with the elution buffer while removed from the magnet and incubated at room temperature for 5 minutes. The sample is then placed in the magnet for 2 minutes before the supernatant is aspirated to a new tube to obtain the extracted nucleic acids.
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Extracting high quality nucleic acids from biological samples is a critical step that must be achieved before any downstream analysis is possible. BioChain specializes in providing a wide variety of easy-to-use and cost-effective extraction kits for this purpose. Bulk quantities and OEM manufacturing are also available for purchase at highly competitive prices.
The company is the world’s best Nucleic Acid Extraction Kits supplier. We are your one-stop shop for all needs. Our staff are highly-specialized and will help you find the product you need.