Background: Phosphatidylcholine injections are becoming an increasingly popular technique to treat localized fat accumulation. This formula is composed primarily of phosphatidylcholine and sodium deoxycholate, a bile salt used to solubilize the natural phospholipid in water. The mechanism through which this injectable phosphatidylcholine formulation causes localized fat reduction is unknown.
If you want to learn more, please visit our website.
Objective: To investigate the active component and mechanism of action of an injectable phosphatidylcholine formulation in clinical use.
Methods: Cell viability and cell membrane lysis assays were performed on cell cultures and porcine skin after treatment with the phosphatidylcholine formula, isolated sodium deoxycholate, or common laboratory detergents Triton-X 100 and Empigen BB. In addition, we described the histologic changes after injection of these substances into porcine tissue.
chenlv Product Page
Results: A significant and comparable loss of cell viability, cell membrane lysis, and disruption of fat and muscle architecture was seen in cell cultures and tissue specimens treated with the phosphatidylcholine formula and isolated sodium deoxycholate. These findings were similar to the effects produced after treatment with laboratory detergents.
Conclusions: The phosphatidylcholine formula popularly used in subcutaneous injections for fat dissolution works primarily as a detergent causing nonspecific lysis of cell membranes. Our findings suggest that sodium deoxycholate is the major active component responsible for cell lysis. Detergent substances may have a role in eliminating unwanted adipose tissue. It is advised that physicians use caution until adequate safety data are available.
Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.Features of Sodium Deoxycholate:• Popular anionic, bile-acid detergent for many laboratory uses• Effective in disrupting and dissociating many types of protein interaction• Can be removed from solution by dialysis• Useful for elution or regeneration of certain kinds of affinity columns• High-purity compound with low UV absorptivity• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt• Molecular Weight: 414.6g• Detergent Class: Ionic (anionic)• Aggregation Number: 5 (average)• Micelle Molecular Weight: 2000g (average)• Critical Micelle Concentration (CMC): 2 to 6 mM (0.083 to 0.249%, w/v)• Cloud Point: Unknown• Dialyzable: Yes• Formula: CNa• Molecular Weight: 414.6g• Purity (by HPLC): ≥98%• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06• pH (1% Solution): 5 to 9• Solubility (in water at 20°C): ≥5%Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ∼2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.
Background: Phosphatidylcholine injections are becoming an increasingly popular technique to treat localized fat accumulation. This formula is composed primarily of phosphatidylcholine and sodium deoxycholate, a bile salt used to solubilize the natural phospholipid in water. The mechanism through which this injectable phosphatidylcholine formulation causes localized fat reduction is unknown.
Objective: To investigate the active component and mechanism of action of an injectable phosphatidylcholine formulation in clinical use.
Methods: Cell viability and cell membrane lysis assays were performed on cell cultures and porcine skin after treatment with the phosphatidylcholine formula, isolated sodium deoxycholate, or common laboratory detergents Triton-X 100 and Empigen BB. In addition, we described the histologic changes after injection of these substances into porcine tissue.
Results: A significant and comparable loss of cell viability, cell membrane lysis, and disruption of fat and muscle architecture was seen in cell cultures and tissue specimens treated with the phosphatidylcholine formula and isolated sodium deoxycholate. These findings were similar to the effects produced after treatment with laboratory detergents.
Conclusions: The phosphatidylcholine formula popularly used in subcutaneous injections for fat dissolution works primarily as a detergent causing nonspecific lysis of cell membranes. Our findings suggest that sodium deoxycholate is the major active component responsible for cell lysis. Detergent substances may have a role in eliminating unwanted adipose tissue. It is advised that physicians use caution until adequate safety data are available.
Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.Features of Sodium Deoxycholate:• Popular anionic, bile-acid detergent for many laboratory uses• Effective in disrupting and dissociating many types of protein interaction• Can be removed from solution by dialysis• Useful for elution or regeneration of certain kinds of affinity columns• High-purity compound with low UV absorptivity• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt• Molecular Weight: 414.6g• Detergent Class: Ionic (anionic)• Aggregation Number: 5 (average)• Micelle Molecular Weight: 2000g (average)• Critical Micelle Concentration (CMC): 2 to 6 mM (0.083 to 0.249%, w/v)• Cloud Point: Unknown• Dialyzable: Yes• Formula: CNa• Molecular Weight: 414.6g• Purity (by HPLC): ≥98%• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06• pH (1% Solution): 5 to 9• Solubility (in water at 20°C): ≥5%Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ∼2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.